We narrowed to 104 results for: control gRNA

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

Curious about what a virus is, and how it differs from a viral vector? Check out our Viral Vectors 101 blog that explains it all!

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C. elegan research.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.