We narrowed to 93 results for: dcas9

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine methylation/demethylation.

CRISPR expression plasmids that have been designed for use in parasites.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.