We narrowed to 97 results for: dcas9

...with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression ...

...CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics...

...regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (CRISPRi), and...

...about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional...

...components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene...

...activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator... iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and ...expression: pAC91 , pAC92 , pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 ...

...modifications at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors...transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used...occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus and its...2016. Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol...

...modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules ...

...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

...serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with...

...Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the MLV Gag ...

... mutations and tags, while a catalytically dead dCas9 can be used in conjunction with activator or repressor...

...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...

...visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or viral-mediated...

...samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR ...

...tools. CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription...

... target RNA without cleaving the molecule (like dCas9) suggests that dCas13a may be useful for isolating...

... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

...researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include...

...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...