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We narrowed to 976 results for: nin

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Showing: 61 - 80 of 976 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...sequence. We have found that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...

  2. CRISPR Guide

    Type
    Collection
    .... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated...in your lab, from knockouts to pooled library screening. Science...CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources ...the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less efficient but high-... , and increases the likelihood that any cell containing the CRISPR plasmid will have all desired genomic...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...

  3. Plan Your Experiment

    Type
    Collection
    ...experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and... are a few factors to keep in mind when designing and cloning your gRNAs. Select Genetic Element to be... into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, the ...your experimental goal, you are ready to start designing the specific components (Cas enzyme and guide ...the next section, we will cover strategies for designing the gRNA component for each category. Genetic ... cells will use error-prone non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity... comes down to location, location, location. Positioning your chosen Cas enzyme at the right spot within...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...220386 Calretinin [L122/132R] Calretinin Human Mouse IgG2a 220387 Calretinin [L122/133R] Calretinin Human...220388 Calretinin [L122/136R] Calretinin Human Mouse IgG2a 220389 Calretinin [L122/20R] Calretinin Human...-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122/...-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122/6R... receptor Rat Mouse IgG2a 114484 Anti-Stonin-2 [N346/9R] Stonin-2 Human Mouse IgG2a 114485 Anti-NSD3 [...

  5. Ras Pathway

    Type
    Collection
    ... RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange...antagonist STK STK3 STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase...small GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve...Rho GTPase activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3...binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene...like RASGRF RASGRF1 RASGRF2 Ras protein specific guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2...member ROCK ROCK1 ROCK2 Rho associated coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor...

  6. Fluorescent Protein Guide: Biosensors

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    Collection
    ...spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin Serotonin (5-HT) sensor...measuring serotonin dynamics. Nat Neurosci. 2021 Apr 5. Yulong Li , J. Julius Zhu Serotonin Serotonin sensor...19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB_5-HT3.0 Dual-color GRAB...Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674...-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery...: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic axon-cilium...calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...Plasmid Backbone Empty backbones are plasmids containing only the elements needed for replication in the...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...start codon for N-terminal tags. And when you are designing your plasmid you should keep your gene "in...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...plasmids for cloning in our Fluorescent Proteins Empty Backbones Collection ). Then, by examining the overlap...Anthony Brown 11908 pEGFP-N1 alpha-actinin 1 Actin filaments alpha-Actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1... Protein PI 71367 Human Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-...Huttenlocher 11908 pEGFP-N1 alpha-actinin 1 Focal adhesions alpha-Actinin 1 EGFP Carol Otey 31923 pPaxillin-PSmOrange...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens junctions beta-Catenin mCherry* Michael Davidson 30313...function. There are a variety of methods for determining subcellular localization of a protein of interest...the cell's internal framework that assists in sustaining structure and function. The cytoskeleton is primarily...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...regions. Blue/white screening can be used for screening of transformants in cloning process. Lynne Postovit...target sites can be inserted into a luciferase-containing plasmid and the effects on gene expression can... of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion of...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly Insertion...

  10. Adenovirus Plasmids

    Type
    Collection
    ...plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene of interest, the ITRs, and homology...the adenoviral plasmid. An adenoviral plasmid containing the rest of the adenoviral genome necessary for...Description PI 16402 pShuttle For production of viruses containing transgene under a chosen promoter Vogelstein ... 16403 pShuttle-CMV For production of viruses containing transgene under CMV promoter Vogelstein 16404...pAdTrack For production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein ...pAdTrack-CMV For production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 50957...RedTrackCMV For production of mRFP-trackable viruses containing transgene under CMV Bamburg 16407 pAdEasy 2-GFP...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ... in one cloning step rather than two. Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. ...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...are mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains (N153AA...fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...

  12. Microbiology Resources

    Type
    Collection
    ...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ....0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : ...predicted open reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate...

  13. Fluorescent Proteins: FRET

    Type
    Collection
    ...their properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor ....8 6.2 3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 ....80 592 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET...maturation time, and other environmental factors. When designing your experiment, be sure to refer to published...mVenus, and Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus...two-hybrid FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart...yield of 93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ..., Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. 2015. An updated ...The CRISPR adaptive immune system seems like a winning strategy to ensure prokaryotic survival, but it...Cas9 could be repaired through non-homologous end joining (NHEJ) , an error-prone process that produces insertions...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. 2015. In vivo genome editing...Zhang F. 2014. Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Science . 343(6166):84-7. PMID...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion... annealed and phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can...Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence...

  16. Synthetic Biology - Overview

    Type
    Collection
    ...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt Clifford Wang Ron Weiss Synthetic... Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic regulatory...

  17. Lentiviral Prep Service

    Type
    Collection
    ...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting...backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting... XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone does not ...backbone pXPR_050 inhibiting 18,901 genes and containing 57,050 unique sgRNAs along with 500 non-targeting...backbone pXPR_050 inhibiting 18,899 genes and containing 57,011 unique sgRNAs along with 500 non-targeting...Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along...

  18. Viral Production

    Type
    Collection
    ...electrophoresis (PAGE) followed by silver staining or SYPRO Ruby staining and the molecular weight and relative...after negative staining. Empty vector particles can be identified after negative staining and appear darker...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative vector preparations...Trono lab . Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation...

  19. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...proteins and empty backbones for expression and cloning of fusion proteins, along with key parameters to...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow...Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD...mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression pFA6a-link-yomCherry-SpHis5...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights...mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep... Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals...and secreted proteins suitable for wholemount immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456...
Showing: 61 - 80 of 976 results