Zhang Lab CRISPR Page
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Collection
...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...sequence. We have found that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...