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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 114 results for: dcas9

Showing: 81 - 100 of 114 results

  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  2. Plan Your Experiment

    Type
    Collection
    ...permanently modifying the genome dCas9-repressor (such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements... elements of target gene dCas9-KRAB is more effective than dCas9 alone for mammalian cell lines. Activate...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter elements...activate or repress a target gene using dCas9-activators or dCas9-repressors, gRNAs should be targeted to...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...

  4. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...

  6. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target...

  7. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...separate accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome...vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Mammalian U6 yes, nick S. pyogenes Conklin pXFokI-dCas9 60901 Mammalian U6 yes, FokI S. pyogenes Conklin...Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236 Mammalian/Lentiviral hU6 yes...Lentiviral U6 none S. pyogenes Puro Zhang pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA 73796 Yeast ...pyogenes Chen pdCas9-DNMT3A-EGFP 71666 Mammalian U6 yes, methylation S. pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR...

  10. CRISPR References and Information

    Type
    Collection
    ...activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator... iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and ...expression: pAC91 , pAC92 , pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 ...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...modifications at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors...transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used...occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus and its...2016. Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  16. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  17. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...
Showing: 81 - 100 of 114 results