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We narrowed to 115 results for: dcas9

Showing: 81 - 100 of 115 results

  1. CRISPR Guide

    Type
    Collection
    ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...imaging of proteins in living cells (Figure 9B) dCas9-VPR - dCas9 fused to several different activation domains...utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin... defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription...target gene CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription...one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene...

  5. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  6. Plan Your Experiment

    Type
    Collection
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ...edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with different base editing...be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene... the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need...N. J. (2023). Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent...

  7. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target...

  8. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  9. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...separate accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome...vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Mammalian U6 yes, nick S. pyogenes Conklin pXFokI-dCas9 60901 Mammalian U6 yes, FokI S. pyogenes Conklin...Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236 Mammalian/Lentiviral hU6 yes...Lentiviral U6 none S. pyogenes Puro Zhang pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA 73796 Yeast ...pyogenes Chen pdCas9-DNMT3A-EGFP 71666 Mammalian U6 yes, methylation S. pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR...

  11. CRISPR References and Information

    Type
    Collection
    ...activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator... iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and ...expression: pAC91 , pAC92 , pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 ...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...modifications at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors...transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used...occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus and its...2016. Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...

  15. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  17. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  18. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  19. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...
Showing: 81 - 100 of 115 results