We narrowed to 213 results for: grna

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

Check out some great blog posts from Addgene Depositors.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Learn about hot new tools in our collection!

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!