We narrowed to 216 results for: grna

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

Check out some great blog posts from Addgene Depositors.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.