We narrowed to 138 results for: yeast

1. Plasmids 101: Blue-white Screening

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   ...Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse All of Our Plasmids 101...

2. Plasmids 101: Mammalian Vectors

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   ...genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...

3. Plasmid Tools for Microbiome Studies

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   ...Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-...

4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... for use in model organisms such as E. coli and yeast. Because this approach uses standardized parts, ...

5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes by El Mouridi...

6. Plasmids 101: Screens vs. Selections

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   ...assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. These involve...

7. GCE4All: Making Genetic Code Expansion Accessible

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   ...techniques being developed. In the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done...

8. RaPID Detection of RNA-protein Interactions

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   ...Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name...

9. Plasmids 101: Modular Cloning

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   ...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made...

10. Plasmids 101: Knockout/Knock-In Plasmids

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    ...constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination...

11. Plasmids 101: Terminators and PolyA signals

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    .... Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion of...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...

13. Depositor Collections

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    ...Collection Promega Plasmid Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration ...

14. Ginkgo Bioworks COVID-19 Collection

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    ...HKU1, and 229E) Expression type (e.g. bacterial, yeast, or mammalian) Tags (e.g. 6xHis, Flag, StrepII, ...

15. Fluorescent Protein Guide

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    ...Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent Protein Collection External Resources...

16. 10 Basic tips for mammalian cell culture

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    ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...comparison to other display systems using phage and yeast (109-1010 members). This selection step involves...

18. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers...

19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring...

20. Plasmids 101: Degron Tags

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    ...It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ...