We narrowed to 147 results for: yeast
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TypeBlog Post...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...
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Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector... marker selection TRP1 Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible...our Plant Plasmids and Resources collection page Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination ...3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag...eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion...C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector that adds a SV40 NLS Myr Membrane... Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...comparison to other display systems using phage and yeast (109-1010 members). This selection step involves... -
10 Basic tips for mammalian cell culture
TypeBlog Post...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don... -
Plasmids 101: Degron Tags
TypeBlog Post...It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers... -
CRISPR Plasmids and Resources
TypeCollection...designed for use in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed...organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...available for many expression systems: bacterial, yeast, and mammalian. These plasmids contain both untagged... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...organisms for expression in prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...open-reading frame (ORF) libraries have been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al... -
Plasmids 101: Codon usage bias
TypeBlog Post...specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and even plants! The zeocin resistance... -
CRISPR Pooled gRNA Libraries
TypeCollection...3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina N/A 1 Varies ...Ebert 3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Editing Prime Editing Other Species Human Mouse Fly Yeast T. gondii E. coli P. aeruginosa S. pneumoniae M.... CRISPRi v1 – barcodes 181005 161769 Inhibition Yeast Ingolia NA ~6 44,580 KSHV Tiling Library 180272 ...Lourido N/A 10 8,158 TUNEYALI Library 217744 Knockout Yeast Borodina N/A 1 56 Turner Lab Human messenger-RBP... -
Pouring LB Agar Plates
TypeProtocol...Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium... -
CRISPR Plasmids - dCas9-FokI
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional...Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Plasmids: Nick Mammalian Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted... Selectable Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable... -
Cloning
TypeGuide...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...