We narrowed to 152 results for: yeast
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TypeCollection... and yeast expression vectors : FX cloning System - Geertsma and Dutzler Labs EasyClone 2.0 Yeast Toolkit...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for ...and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for...-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common...depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The ...Link opens in a new window) National Collection of Yeast Cultures (NCYC) (Link opens in a new window) NIH...
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Pouring LB Agar Plates
TypeProtocol...Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...available for many expression systems: bacterial, yeast, and mammalian. These plasmids contain both untagged... -
Plasmids 101: Codon usage bias
TypeBlog Post...specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...organisms for expression in prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...open-reading frame (ORF) libraries have been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and even plants! The zeocin resistance... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression...Zebrafish/Xenopus/C.elegans/Sea urchin pKT0128 - Yeast Expression EGFP-pBAD - Bacterial Expression mEGFP... Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K) dClover2...Expression mVenus C1 - Mammalian Expression pKT0090 - Yeast Expression mVenus-pBAD - Bacterial Expression moxVenus...Expression mGold 515 530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mCitrine...Mammalian Expression pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1...Mammalian Expression pFA6a-link-yomRuby2-SpHis5 - Yeast Expression mRuby2-N1 - Mammalian Expression mRuby2... -
CRISPR Plasmids - Yeast
TypeCollection... CRISPR Yeast CRISPR Plasmids: Yeast Browse CRISPR Plasmids By Function...have been designed for use in yeast. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...ability to invert or ‘flip DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). ... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post... types including (but not limited to) bacteria, yeast, plants, drosophila, worms and mammals (Browse plasmids... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post.... These are less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species. ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post... unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...expression in mammalian systems, bacteria, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid... S. pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector... marker selection TRP1 Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible...our Plant Plasmids and Resources collection page Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination ...3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag...eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion...C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector that adds a SV40 NLS Myr Membrane... Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable...