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Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

Curious about what's new in the Addgene repository this spring? Check out our latest Hot Plasmids post!

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

Zebrafish larvae is a great model for studying development and embryogenesis. Learn more about genetic tools for zebrafish in this blog post.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Learn about hot new tools in our collection!

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

If you need to image more than one protein in your sample, multiplex immunofluorescence is an important skill to master!

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Learn more about the common E. coli strains used in the lab.

Learn about fluorescent protein techniques you can use to monitor cellular trafficking throughout an organism.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.