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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 212 results for: FAST

Showing: 101 - 120 of 212 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ...ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post...

  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation)...and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them for a...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...gene that is shared across all CRISPR types; the fast evolution of these adaptive bacterial and archaeal...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab...cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression...N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector ...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... FUS_LC_withR FUS T7 ALS Nicolas Fawzi 139211 pFastBac1-LRRK2 WD40 LRRK2 His, MBP polH Parkinson's Hao...S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker...6His HTRA2 His T7 Parkinson's Eric Fischer 198033 pFastBac-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10...P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha ...Sascha Martens 198072 pFastBac_Dual_GST-TEV-EGFP-TBK1deltaCTD (res 1-666) TBK1 GFP, GST, Tev P10 ALS Sascha...) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens...PARK7 mTagBFP2 CMV Parkinson's Timothy Ryan 221331 pFastBac-GST-TBK1 TBK1 His, TEV, GST polH ALS Jonathan ...
Showing: 101 - 120 of 212 results