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We narrowed to 212 results for: FAST

Showing: 101 - 120 of 212 results

  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ...ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation)...and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them for a...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...gene that is shared across all CRISPR types; the fast evolution of these adaptive bacterial and archaeal...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab...cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression...N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector ...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....
Showing: 101 - 120 of 212 results