We narrowed to 268 results for: aav
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TypeBlog Post...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...
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Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1! New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab. vfCRISPR...Gether lab, now available as ready-to-use AAV vectors. ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep. Flp-dependent DREADDs from the Gether... -
Reflections and Looking Ahead
TypeBlog Post...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged... -
Hot Plasmids - October 2020
TypeBlog Post... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider... -
Sensing Neuronal Dopamine
TypeBlog Post...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila... -
Meet the New Addgene Board Members!
TypeBlog Post...project to develop an AAV-based COVID-19 vaccine. Vandenberghe’s work with AAVs led to many interactions...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post...Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once... -
8 Great Depositor Research Stories
TypeBlog Post...a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research... excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs ...associated with AAV; co-infection with both constructs produces a minimum of 8 colors. The use of AAV provides... -
Viral Vectors 101: Transductions
TypeBlog Post...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned... -
New Viral Vectors - Summer 2024
TypeBlog Post...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)? AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to create both transient cell...You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...here. AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in... -
Plasmids 101: FLEx Vectors
TypeBlog Post...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...cells, commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver...