Addgene

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Showing: 101 - 120 of 153 results

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Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

...fusions to connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as...
Transferable Skills Guide: Cross-team Communication

Type

Blog Post

...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

...predecessor. Important features may include cloning junctions (where most sequence assembly errors occur), deletions...
The Future of Research Symposium Boston 2015

Type

Blog Post

...Shaping the Future of Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID...
Plasmids 101: Cre-lox

Type

Blog Post

...alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

... vectors Behavioral testing is often done in conjunction with a genetic manipulation so that behavior ...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

...Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule FRET for understanding biomolecular...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...Shaping the Future of Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID...
Hot Plasmids February 2024

Type

Blog Post

...cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the...
Gaining Leadership Skills Volunteering at a Professional Organization

Type

Blog Post

...improving my skills and sharing with other more junior leaders what I had experienced and learnt. Opening...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity...
PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

...clones displayed proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...while a catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of ...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated total ...
Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

...., Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell...
MXS Chaining

Type

Blog Post

...acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810. ...
Lentiviral Vector Uses and Overview

Type

Blog Post

...RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb;15...
Plasmids 101: Colony PCR

Type

Blog Post

... insert orientation, and the sequences of the junctions between the plasmid and insert DNA. Colony PCR...

Showing: 101 - 120 of 153 results

Results per page:

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Transferable Skills Guide: Cross-team Communication

Addgene's Tips for Plasmid Quality Control

The Future of Research Symposium Boston 2015

Plasmids 101: Cre-lox

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Fluorescent Proteins 101: Introduction to FRET

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Hot Plasmids February 2024

Gaining Leadership Skills Volunteering at a Professional Organization

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

PITChing MMEJ as an Alternative Route for Gene Editing

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Course-Based Undergraduate Research Experiences (CUREs)

MXS Chaining

Lentiviral Vector Uses and Overview

Plasmids 101: Colony PCR

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