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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 212 results for: FAST

Showing: 121 - 140 of 212 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... FUS_LC_withR FUS T7 ALS Nicolas Fawzi 139211 pFastBac1-LRRK2 WD40 LRRK2 His, MBP polH Parkinson's Hao...S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker...6His HTRA2 His T7 Parkinson's Eric Fischer 198033 pFastBac-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10...P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha ...Sascha Martens 198072 pFastBac_Dual_GST-TEV-EGFP-TBK1deltaCTD (res 1-666) TBK1 GFP, GST, Tev P10 ALS Sascha...) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens...PARK7 mTagBFP2 CMV Parkinson's Timothy Ryan 221331 pFastBac-GST-TBK1 TBK1 His, TEV, GST polH ALS Jonathan ...

  2. Lentivirus Plasmids

    Type
    Collection
    ...selection markers. Tobias Meyer 86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    .... This method of cell lineage conversion can be faster, bypasses intermediate pluripotent stages, and ...

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave or...

  6. CRISPR Library Amplification

    Type
    Protocol
    ... that contain a recombinant plasmid likely grow faster in the closed system of a crowded plate or culture...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

  8. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    ...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

  10. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  11. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... viral DNA for quality control. This gives us a faster turnaround time than short-read sequencing, as ...The workflow converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools...program called Geneious. Within Geneious, the raw FASTQ reads are used for either guided or new de novo ...

  14. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative...30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially...

  15. Summer SciComm: Preprints

    Type
    Blog Post
    ...pandemic quickly and openly. By sharing results faster and more broadly, scientists can receive recognition...Preprints are all about sharing scientific knowledge faster and more openly — which is also our mission here...

  16. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...to mankind. And you probably understand how much faster it would be for an expert in the field to read ...students were fired up and enthusiastic and needed a faster presentation with more information than normal....

  18. Pooled Library Amplifications

    Type
    Blog Post
    ...smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts of... with a single size insert, liquid media can be faster and easier to work with. In either case, it can...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...bacterial and yeast cells are cost-effective and faster-growing than mammalian cells, but mammalian cells...successful.  Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ...syringe filter method is not only cheaper, but also faster and more flexible than commercial kits.  DNA gel...bead-based kit, this cellulose-based method was faster, cheaper, and had a similar level of sensitivity...
Showing: 121 - 140 of 212 results