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Showing: 121 - 140 of 1033 results
  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Protein PI 71367 Human Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens junctions beta-Catenin mCherry* Michael Davidson 30313...Fluorescent Protein Collection or search our full catalog for a marker for your structure of interest. Additional...
  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...programming sgRNA and HR donors for selectable genome modification. This system can be used to create C- and N-...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...
  3. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs.... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...
  4. Tags and Other Markers

    Type
    Collection
    ...to find antibodies currently available from our catalog that target epitope tags and other common cellular...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...
  5. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...
  6. Neuroscience

    Type
    Collection
    ...to find antibodies currently available from our catalog that target proteins popular in neuroscience research...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...
  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...
  8. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... variation. These joint efforts will produce a catalog of the impact of genomic variants on genome function...Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF Consortium (Link...opens in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...
  9. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...
  10. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...experiment. Browse Fluorescent Protein Plasmids by Category Empty Backbones Study your protein of interest...
  11. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Visualize Genomic Loci Mammalian Resources Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...
  12. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...
  13. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...
  14. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...
  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...
  16. Synthetic Biology - Overview

    Type
    Collection
    ... by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including... , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and...
  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ...
  18. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...
  19. Viral Production

    Type
    Collection
    ...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...
  20. Lentivirus Plasmids

    Type
    Collection
    ... plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression...monitor expression. Luk Parijs 11619 pLB 3rd Modification of pLL3.7 with added genetic elements known ...3rd For barcoding transcription factors for identification in pooled screens. Part of the MORF Collection...
Showing: 121 - 140 of 1033 results