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We narrowed to 626 results for: %s

Showing: 161 - 180 of 626 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where ...new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein that is smaller and easier...pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng'...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al. Systematic profiling of conditional degron...Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific...proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: https://doi.org...

  4. Typing CRISPR Systems

    Type
    Blog Post
    ....2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou,... Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier...H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu, F. E., Moeller, L., Kocalar, S., Oshiro, R...Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong, S., Makarova, K. S., Koonin, E. V., Cheng, D. R...References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns..., D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P....

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... because I only left him/her with small tasks and s/he was always asking for more work (this is a big ...your network…. I found it helpful to plan my week(s) in advance as much as possible so I could more easily...support you, just like you are supporting your report(s). This means that you should be able to go to your...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... Hao, Y. A., Lee, S., Roth, R. H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure ...doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein...synthetic biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

  8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for ...Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens...Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve...1985; 43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...membrane protein interactions. Proc Natl Acad Sci U S A. 2004; 101(33):12242–12247. Pubmed PMID: 15299147...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: The...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...processed miRNA or siRNAs then silence the target gene(s) either transcriptionally or post-transcriptionally...produced that contains sequence against your gene(s) of interest and the reporter. This approach greatly...Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack...F, Joberty G, Zinn N, Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, ...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM, Younger... Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence...10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak...

  13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction Efficiency, Tropism... OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant....org/10.1016/j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette...

  14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...detectable using PCR. The system also functions in S. pneumoniae and can be used to generate multiple mutations...gene lambda Red. pTargetF contains the specific gRNA(s), and the repair template is supplied as a dsDNA fragment...Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE (2018....7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-...AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating essential gene function...

  15. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene....the most frequently used yeast strains: S. pombe (fission) and S. cerevisiae (budding). Yeast make for ...probably wondering if the “biotech yeast” is S. pombe or S. cerevisiae. Actually, it’s neither. The most...phaffii and other yeast strains.     S. cerevisiae S. pombe K. phaffii Growth properties ...glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily it evolved much... K. phaffii to work   The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere anytime soon...Amenable to genetic manipulation The first eukaryote (S. cerevisiae) to have its genome fully sequenced There...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou,...Toh, M. S., Irby, M. J., Edwards, L. S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019...Cas3’s degradative power in the future! References and Resources References Loeff, L., Brouns, S. J.,... H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T....x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D., Rotstein...Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns...through the Cas3. This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff, making you into...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, for mouse or human receptors, respectively). ...AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances in...

  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated...doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein as a Solubility Enhancer...
Showing: 161 - 180 of 626 results