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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: (3-F)Tyr-EGFP

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Showing: 1 - 3 of 3 results

  1. Genetic Code Expansion

    Type
    Collection
    ...nitroY/haloY-F5 PylRS M. alvus 3-nitrotyrosine (3-nitro-Y), 3-halotyrosine (3-halo-Y) Bacterial TAG Ryan ...nitroY/haloY-F5 PylRS M. alvus 3-nitrotyrosine (3-nitroY) and 3-halotyrosine (3-haloY) Bacterial Ryan Mehl...MfnG-EcYRS-EGFP* TyrRS E. coli O-methyltyrosine (O-Me-Tyr) Mammalian TAG Han Xiao 193250 pMV361_MjTyrRS tyrosyl-tRNA... Rinehart 191118 pCS2-EcYRS-EGFP* TyrRS E. coli O-methyltyrosine (O-Me-Tyr) Mammalian TAG Han Xiao 191119...Mehl 85498 pDule-3-nitroTyrosine (5B) 3NY (5B) synthetase M. jannaschii 3-nitroTyrosine Bacterial TAG Ryan...Mehl 85499 pDule2-3-nitroTyrosine (5B) 3NY (5B) synthetase M. jannaschii 3-nitroTyrosine Bacterial TAG Ryan...jannaschii 3-aminotyrosine Bacterial TAG Huiwang Ai 153558 pMAH-EcaYRS EcaYRS E. coli 3-aminotyrosine Mammalian...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR...) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains...PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126/science.1231143. PubMed ....efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A ...SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro...) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single guide RNA 62987 : PX462...

  3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...three inactivated eGFP reporters- eGFP L202, eGFP L138, and eGFP L93. The modified eGFPs were placed downstream...comparing cells expressing only eGFP expression to cells expressing eGFP and mCherry. ACE was used to identify... To create the eGFP reporters the Harris lab individually mutated three codons in eGFP to eliminate fluorescence...editisome complexes. The eGFP reporters rely on the correction of point mutations in eGFP that results in the...mutated mCherry and a downstream constitutively active eGFP. To create the inactive mCherry, the lab introduced...fluorescence to monitor APOBEC-Cas-9 mediated editing. eGFP reporters for single base editing by APOBEC-Cas9...previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target DNA editing ...
Showing: 1 - 3 of 3 results