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We narrowed to 253 results for: CIL

Showing: 1 - 20 of 253 results

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that allows ...lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 colonies...all steps consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used... per well for a final volume of 150 μl. This facilitates screening by allowing a multichannel pipette ...

  4. Viral Production

    Type
    Collection
    ...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...cholecystokinin interneurons in the basal amygdala facilitates fear extinction. eNeuro. 6(6). PubMed (Link opens...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

  7. Synthetic Biology - Overview

    Type
    Collection
    ...leading synthetic biologists around the world to facilitate the sharing of parts and information. Addgene...Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt...

  8. Microbiology Resources

    Type
    Collection
    ...islandicus Plasmids for Bacteria Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter...sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori...

  9. AAV Packaged on Request

    Type
    Collection
    ...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...all-inclusive of several important steps: MTA facilitation for the transfer plasmid, including legal approval...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...apyrimidinic (AP) site. UNG / UDG Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site... Editors. CGBEs first convert a cytosine (C) to uracil (U), just like Cytosine Base Editors (CBEs). But...empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other ...between the pyrimidine bases (cytosine, thymine, and uracil). Base transversions are edits that convert a purine...leads to the first adenine transversion editors Uracil excision is the most common type of base excision...perhaps analogous engineering of UNG, which excises uracil, could allow it to recognize thymine (T) or cytosine...CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase to a CBE; the first type of base...

  11. Open Enzyme Collection

    Type
    Collection
    ...marker for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols...BGT beta-glucosyltransferase 165531 pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...selection curve below. Carbenicillin is used here in place of ampicillin because carbenicillin is more stable,...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn...Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin stock used...protocol will focus specifically on selection with carbenicillin; however it can be adapted for any antibiotic...antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic...coli transformed with a plasmid containing the carbenicillin (amp) resistance gene (or other antibiotic resistance... resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4 mg/mL in LB medium...

  13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...(step 2 and 3). Then the uracil-labeled template strand is degraded by uracil DNA glycosylase (UDG) and... in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes...removes uracil from newly synthesized DNA. Lack of both enzymes leads to a buildup of uracil and the insertion...Supplementary Table 1 of Wrenbeck et al). It uses a uracil labeled wild-type ssDNA as template for a site-...Exonuclease III (ExoIII) (step 4). UDG recognizes uracils that are in DNA and ExoIII recognizes nicked duplexed... insertion of uracil into newly synthesized DNA. This labeled ssDNA is packaged into phage and then extracted...taking a couple days to generate the phage-derived uracil labeled template, this method requires further ...

  14. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...environments.   Carbenicillin   Carbenicillin, colloquially known as carb, belongs to the penicillin family of...issue. The genes and their antibiotics   Ampicillin Ampicillin, often just called amp, is the historical...derivative of the more widely known antibiotic, penicillin, and is a beta-lactam that inhibits cell wall...protein synthesis. The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it.... with most bacteria? No satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name...purpose="decorative" title="Check icon" %} Carbenicillin Excellent {% icon icon_set="fontawesome-5.14.0...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... / UDG Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. UGI Uracil Glycosylase...removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil editing...between the pyrimidine bases (cytosine, thymine, and uracil). Base transversions are edits that convert a purine... editors, like calling it the “edited strand.” Uracil excision repair leads to the first base transversion...cytidine (C) deamination, converting the C base to uracil (U). This is the same chemical reaction performed...editing, and instead introduced its opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting...Glycosylase Inhibitor Inhibits UNG to preserve uracil bases in DNA. References Chen, L., Park, J. E., Paa...

  16. COVID-19 Resources

    Type
    Collection
    ...cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-2 infection induces a... - Kogiso H, et al, 2020, Int J Mol Sci Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal...Bioanalytical: #AB01200-02000 Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use ...from each ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and...and cool to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100...tract, cPPT, improves transduction efficiency by facilitating nuclear import of the vector’s preintegration...ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid...#11995 Fetal Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene...

  18. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...colonies! Carbenicillin is more stable than Ampicillin and can be used in place of Ampicillin in most applications...protein synthesis Bactericidal 25-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal...Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal 100...most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast and plates should...

  19. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...to the United Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on...mitochondrial diseases. The key success of the Nuffield Council’s report is that when it became clear that this ...transmission of mitochondrial disease;” The Nuffield Council began their research into both novel and previously...government regulators, and an independent ethics council – all worked in tandem, within their respective...germline editing technique—too. As the Nuffield Council emphasized, the key to moving research from bench...

  20. The MTA at Addgene

    Type
    Blog Post
    ...MTAs.  Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is important...deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs with 8,798 organizations in 111 ...111 countries. From 2019 to 2023, we averaged facilitating 89,477 MTAs per year.  The MTA Process at Addgene...MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by the Provider and Recipient...signature on any subsequent MTA for that plasmid facilitated by Addgene — basically, instead of having to ...
Showing: 1 - 20 of 253 results