We narrowed to 19 results for: Lime

1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   ...quantum yield, and expression. Create yellow (v82) and lime green (v857) variants differing in kinetics, affinity...through two different variants The yellow (v82) and lime green (v857) versions can be summarized quickly ...

2. Synthetic Biology - Assembly Standards Guide

   Type

   Collection

   ...meaning that the suffix will need to be reverse complimented for primer design. The restriction enzymes used...

3. Tag Your Favorite Yeast Genes with Ease

   Type

   Blog Post

   ...GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with...

4. In Living Color: The Skinny on In Vivo Imaging Tools

   Type

   Blog Post

   ...higher resolution images at depths up to eight millimeters – not single cells (yet) but small clusters of...

5. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   ...deoxyadenosine (A) at the 3' ends of PCR products. The complimentary “T” in the pair comes from a topoisomerase I-linearized...

6. Advice for Moving Into Sales After Your Science Postdoc

   Type

   Blog Post

   ...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...

7. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   ...treatment and gene therapy have also been in the limelight. The FDA approved the first oncolytic viral therapy...

8. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   ...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

9. Working with Nuclear Receptors

   Type

   Blog Post

   ... the current phase of NURSA we are seeking to compliment these consortium-generated datasets with a systematic...

10. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    ...asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple, and...

11. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...slow and unwieldy for large specimens that are millimeters in thickness (8,9).  In addition, there is a ...

12. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ...now, 2 decades later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna...

13. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    ...feel comfortable speaking on topics that would compliment the STEM curriculum. As it happened, I’d met ...

14. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ..., instead of being 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way ...

15. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    ...of inhibition around the disk is measured in millimeters, and corresponds to the level of susceptibility...

16. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    ... this system to be used to trace neurons over millimeter-long distances across multiple fields of view...

17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    ...allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons (typically from...

18. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ...original reference library, as well as GC%, reverse compliment sequences, and more. Output_Compact: A text file...

19. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

    ...CAATTG ). The bottom oligo will be the reverse compliment so that they can anneal. Top oligo: 5' - CATATG...