...complex (LY6A) as a receptor for PHP.B. Read on to learn how the design of PHP.B may have contributed to this...receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by PHP.B viruses...viruses. Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J mice is likely due to...the Deverman and the Gradinaru labs engineered PHP.B. PHP.B was created by first generating a diverse library...version of PHP.B, shows similar transduction efficiency, but at a log lower dose than PHP.B.  A Ly6a variant...CNS was transduced by PHP.B but absent in mice who were not transduced by PHP.B. The Deverman Lab took... non-permissive to PHP.B CNS transduction. Two genes were associated with the PHP.B permissive phenotype...

...calling “strain-specific tropism.” For example, AAV-PHP.B doesn’t lead to enhanced transgene expression, compared...strain in which the capsid was first selected. AAV-PHP.B, and many capsids derived from it, cross the BBB...Mouse Strain-Specific Tropism?  Reference AAV-PHP.B* CNS (broad) Mice, rats Yes (LY6A) Deverman...attributed were deposited by the Gradinaru lab.  AAV-PHP.B was the first systemic capsid developed and deposited...relative to AAV9. At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce...J. M. (2018). The Neurotropic Properties of AAV-PHP.B Are Limited to C57BL/6J Mice. Molecular Therapy,... (2019). The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Molecular...

...of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or an ....eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse...Transduced   Cortex Striatum Cerebellum AAV.PHP.B (parental) 49 +/- 7% 36 +/- 8% 37 +/- 10% AAV.PHP.eB...

...PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038...