We narrowed to 13 results for: PMD
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TypeBlog Post...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has ...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...effects. To date, nearly 900 articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one ...one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the... pmd2.g...
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15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope...’s meant to be used with pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from ...plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from...the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from ... -
Viral Production at Addgene
TypeBlog Post...in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell... -
Hot Plasmids: Spring 2025
TypeBlog Post...generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). ... -
Plasmids 101: Dimers and Multimers
TypeBlog Post... Fig. 1: The monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The... -
Lentivirus Plasmids
TypeCollection...deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be ...and 3rd generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid... -
Retrovirus Plasmids
TypeCollection...protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono... -
Viral Production
TypeCollection...in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . ... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (... -
Lentivirus Production
TypeProtocol...Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro...