... (red) 2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent....7 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465...separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue...2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 ...monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach et al.) were derived from previously...Subach et al made a concerted effort to develop their FT series using their knowledge of protein structure...mutagenesis. mK-GO matures from green to red, and the FT series matures from blue to red (see Table 1 for ...

...high blue to red ratio, whereas cells with long or slow cycles are more red than blue (Eastman et al., 2020...University fused a color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation...conformational change causes the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half ... Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3...acute myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across...across these different cell types. In one example, H2B-FT was expressed in murine B cells (BaF3), which depend...

...their activation. The four main FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift...switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers (FT): These proteins change their color over time. Therefore...

...Monomer pSlow-FT-N1 - Mammalian Expression pTRE-Slow-FT - Mammalian Expression pBAD/HisB-Slow-FT - Bacterial...Monomer pFast-FT-N1 - Mammalian Expression pTRE-Fast-FT - Mammalian Expression pBAD/HisB-Fast-FT - Bacterial...pMedium-FT-N1 - Mammalian Expression pTRE-Medium-FT - Mammalian Expression pBAD/HisB-Medium-FT - Bacterial...Bacterial Expression Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (...Brightness pKa Maturation Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue...Bacterial Expression Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 hr ...