Hot Plasmids February 2024
Type
Blog Post
...Peptide-assisted genome editing for primary cells
Monomeric StayGold variants
AAV.CAP-Mac for systemic gene transfer...37095348.
Breaking up is hard to do: Monomeric StayGold variants
By Mike Lacy
In 2022, Atsushi Miyawaki... highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide interest, its dimeric...
The Miyawaki lab generated a series of StayGold mutants and identified a triple mutant, Y187F,...Balasubramanian and colleagues showed the single mutation E138D is sufficient to disrupt dimerization while maintaining...and colleagues generated yet another monomeric StayGold variant (Piatkevich et al., 2023). Check out their...Addgene here!
Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications...