We narrowed to 7 results for: allelic exchange

...technique is frequently called allelic exchange. Here, a mutated allele introduced from a conjugative ... Continued development of new allelic exchange vectors Allelic exchange is a great technique for the scarless...off-target mutations. Ultimately, successful allelic exchange requires two sequential homologous recombination...counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB, an chloramphenicol...resistant colonies isolated are those where the allelic exchange vector has undergone single crossover and ...develop these negative selection markers for allelic exchange. To do this, we performed the following modifications...blue or magenta, they are real! Planning an allelic exchange experiment? Find the pTOX plasmids here! Continued...

...specific purposes.     Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP...chromoproteins to create negative selection markers for allelic exchange. These vectors contain a toxin gene (regulated...rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange occurs through two sequential homologous recombination...encode the tsPurple chromoprotein.  Find the allelic exchange vectors here! ShadowR, a novel FLIM-FRET acceptor...M., & Waldor, M. K. (2019). A New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial...

...for many molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work ...

...-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional alleles in mouse embryonic ... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...

...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...Figure 4: A targeting vector to create a floxed allele for a conditional knockout. This experimental design...include loxP sites to create conditional floxed alleles. Although CRISPR is very good at making knockouts...

... could recapitulate or rescue known pathogenic alleles, or introduce a premature stop codon to render ... their RESCUE (RNA Editing for Specific C-to-U Exchange) system (Abudayyeh et al., 2019). To avoid some...

...sites, and provides a modular system for easier exchange and re-use of existing elements. DNA sequences...expression. The capability to turn RUSH and CRUSH alleles off or on enables this method to rapidly address...carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and clonogenic...