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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: firefly luciferase

Showing: 1 - 9 of 9 results

  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter.... Figure 1: Firefly, from Wikimedia Commons. The North American firefly, Photinus pyralis, ...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...Direct injection of plasmids for Antares or firefly luciferase into mouse livers followed by i.v. injection...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits ...

  3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ... of viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify... a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene...template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating ORF-Firefly luciferase...118017 pLX313-Firefly luciferase Firefly EF1α Lentiviral expression of firefly luciferase William Hahn,... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of interest...pGreen2x35SLUC Firefly, Renilla Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under... pGWB-nLUC Firefly Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation...w567-1) Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly...

  5. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...et al. Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...normalized to assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...

  9. Bacterial Expression Systems

    Type
    Collection
    ...pMV306hsp+FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control of...
Showing: 1 - 9 of 9 results