...This is the fifth in a 5 part series on Management for scientists.  We are inundated with articles and...leader-specific. How is leadership different from good management? Aren't all career skills leaderhsip skills when...interview with Connie Cepko to learn about her management style. Leaders look to the future Leaders must... blog posts in one pdf - download the Addgene Management for Scientists Ebook.  Fire, Snowball, Mask:... management for scientists...

...This is the fourth in a 5 part series on Management for scientists. Once you are responsible for managing...School researcher Connie Cepko to learn about her management style. Recipes for delegation success Remember...Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How Volunteering ...  management for scientists...

...This is the third in a 5 part series on Management for scientists. Feedback is help and it should result...School researcher Connie Cepko to learn about her management style. Formalizing the feedback session Giving...routines. This is the third in a 5 part series on Management for Scientists. Subscribe to the Addgene Blog... management for scientists...

... part series on Management for scientists. This second installment in the Management for Scientists series... – getting feedback from the team. Successful management can almost be boiled down to one, key concept... is important because it creates a culture of engagement in the issues at hand and eagerness to get, as...School researcher Connie Cepko to learn about her management style.   Seeking feedback frequently One of the... management for scientists...

... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab... and paper will fail you. The benefits of lab management software aren’t just limited to large volume ...

... Today in our transferable skills guide: Time Mangement for Scientists. Back when I used to do mammalian...the lab. Obviously I didn’t have the best time management skills, but I’ve learned a lot since my days ... probably already started coming up with time management techniques of your own, but if you ever find ...about my own processes, I’ve broken down time management into three conceptual chunks: prioritization,...prioritization, planning, and procrastination. Time management through prioritiziation Every time you begin a task,...with friends by splitting my cells first. Time management through planning I probably wouldn’t have fallen...projects more manageable. If you have any other time management suggestions, please be sure to note them in the...

... 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does GEmCherry2 work? The...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems...fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair editing CRISPR...genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein for...monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9...and activity (Knudsen et al., 2018).  To create GEmCherry2, the Denham lab first incorporated an out-of-...methionine start site. This first iteration was named GEmCherry1, due to the “genomic fragment inserted into mCherry...

...guide you through the basics of CombiGEM-CRISPR experiments. CombiGEM-CRISPR: The marriage of two simple...gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal ... complex regulation in biological systems. The CombiGEM platform provides a means to create barcoded gRNA..., 2014; Wong et al., 2015; Wong et al., 2016). CombiGEM uses iterative one-pot reactions with straightforward...quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can accommodate any genetic...address the users’ specific research questions. CombiGEM has been successfully applied to functionally ...

...software product management, I confess that I had no idea what software product management was either. Coming...and begin my product management (PM) career. Had I known about product management earlier on in my career...software product management. Early in your career: Try to get software product management internships, preferably...do. Fortunately, the head of software product management at my company was open to meeting with me and...they will be happiest. What is software product management, and how does it relate to science? In the simplest...What type of person goes into software product management? I always tell people the best part of my job...great solution.  How to get into software product management? Depending on the stage in your career, there...

... This is the first in a 5 part series on Management for scientists.  “I'm slowly becoming a convert ...You can avoid some of these common pitfalls of management and still not get the most from your team. It... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their... management for scientists...

...approach to management and the importance of realizing that different people have different management needs...needs. How Crystal developed her management skills - training, learning from peers, and being receptive to...  Additional Resources from Addgene Find more management resources in our Mentoring for Scientists Series...

... Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest, Tom Ellis...directly involved in supervising many successful iGEM undergraduate projects. Special thanks to our sound...

...flame mitochondria-targeted calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor...

...Restriction Sites to Avoid: NotI For more info, visit iGEM: BioBrick (RFC 10) Back to Top BioBrick BB-2 (RFC...PvuII, XhoI, AvrII, XbaI, SapI For more info, visit iGEM: BioBrick BB-2 (RFC 12) Back to Top BglBrick – Berkeley...: EcoRI, BglII, BamHI, XhoI For more info, visit iGEM: BglBrick – Berkeley Standard (RFC 21) Back to Top...Sites: EcoRI, XbaI, SpeI, PstI For more info, visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard...XbaI, NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...

...Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use Addgene's curated tables...The schematic below shows the three types of rearrangements: inversion, deletion and translocation. Inversion...chondrocytes Xenopus Col2a1 Vertebrate Tanaka 111153 pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert mCherry...hsp68 T. castaneum (red flour beetle) Averof 119868 pGEM-NLS-Cre-2A-mCherry-FKF NLS-Cre, mCherry T7 Bacterial...

...Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-...Effectors Zinc Finger Consortium Community Resources iGEM - International Genetically Engineered Machine Foundation...

...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...

...codon-optimized Cas9 nuclease expression plasmid. The arrangement of the Nuclear Localization Signal (NLS) relative...

...voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to...

...email you a link to the Recipient Scientist Acknowledgement (RSA). This form can be completed electronically...