We narrowed to 7 results for: mK-GO
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TypeBlog Post...propensity to aggregate. These monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach...mCherry, respectively. While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance...of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT series matures...membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing...
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Plasmids 101: Common Lab E. coli Strains
TypeBlog Post... hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA hsdS(rk-,mk-) Unmethylated... nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– EPI300 Cloning large inserts and transformation... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) JM110 Streptomycin For storing plasmids that... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) MC1061 Streptomycin Parent of DH10B/TOP10 and...glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+) XL10 Gold Tetracycline and Chloramphenicol High...post describing protein expression strains, where we go over the basics of protein expression in E. coli ...Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101... -
Summer SciComm: Preprints
TypeBlog Post...strategies for your scientific communication needs. Going to present some unpublished results at a conference...preprint, further increasing their findability. Now, go out there and finish that experiment (and don’t forget.../preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...team at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems...pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019) Segmented poly(A) tails significantly... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after... -
Molecular Biology Reference
TypeGuide...Phi80lacZDeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC ...Promega e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Delta(lac- proAB) [F traDelta36 proAB...Replication Plasmids 101: The Promoter Region-Let’s Go Promoters Reference Page Plasmids 101: How to Name... -
Immunology Research Plasmids and Resources
TypeCollection...Gene Lists from Immport . Click the links below to go to the gene table for each category. The gene names...midkine (neurite growth-promoting factor 2) FLJ27379, MK, NEGF2 MET met proto-oncogene (hepatocyte growth ...