...propensity to aggregate. These monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach...mCherry, respectively. While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance...of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT series matures...membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing...

... hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– HB101 Streptomycin Hybrid of E. coli K12 ... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) JM110  Streptomycin  For storing plasmids that... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)  MC1061 Streptomycin Parent of DH10B/TOP10 and...glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High...post describing protein expression strains, where we go over the basics of protein expression in E. coli ...Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101...

...strategies for your scientific communication needs. Going to present some unpublished results at a conference...preprint, further increasing their findability. Now, go out there and finish that experiment (and don’t forget.../preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (...

...team at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems...pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019) Segmented poly(A) tails significantly...

...FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after...

...Phi80lacZDeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene...Promega e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Delta(lac- proAB) [F traDelta36 proAB...Replication Plasmids 101: The Promoter Region-Let’s Go Promoters Reference Page Plasmids 101: How to Name...

...Gene Lists from Immport . Click the links below to go to the gene table for each category. The gene names...midkine (neurite growth-promoting factor 2) FLJ27379, MK, NEGF2 MET met proto-oncogene (hepatocyte growth ...