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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15 results for: pCMV-HA

Showing: 1 - 15 of 15 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    .... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the...Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use...

  2. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned... CRISPR has made it possible to design programmable epigenome editing tools, and the field has certainly...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...have available by category. Each plasmid page now has a helpful graphic to introduce you to the plasmid... the Transcriptional Regulation category already has five blue flame plasmids covering three different...LwCas13a guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing: Did anyone...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ... Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled...1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Mitochondria-Outer Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois...anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois... Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois... protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion proteins...

  5. Lentivirus Plasmids

    Type
    Collection
    ...plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,...virus integrates randomly into the host genome, it has the potential for insertional mutagensis. The 2nd...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget...enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small...

  7. Genetic Code Expansion

    Type
    Collection
    ...164080 pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS...164195 pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS...SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403 pCMV-DnpK PylRS M. barkeri N6‐(2‐(2,4‐dinitrophenyl)acetyl..., DiZSeK, or DiZHSeC Bacterial TAG P. Chen 91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids...aminoacylate the orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

  8. Retrovirus Plasmids

    Type
    Collection
    ...pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging... expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000...shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer that is released by digestion with... the sequencing conditions if the DNA polymerase has difficulty reading through the secondary structure... in contact with the walls of the tube before it has been diluted. Mix by swirling or gently flicking ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase...of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly...and Luciola luciferase . Each type of luciferase has advantages and disadvantages depending on the application...expression Renilla luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    .... Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs...sum of epigenetic modifications across the genome) has been implicated in disease states, and targeting ...genomic loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics began...many TALEN-based technologies, the advent of CRISPR has made targeting much easier! With the help of guide...modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including...BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created pdCas9-Tet1-CD for targeted cytosine demethylation...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...MbPyl pyrrolysyl-tRNA synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements...fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed a synthetic red fluorescent protein... imaging and in protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. ...efficiency Blue flame depositor Yannick Doyon’s lab has developed a new method to increase CRISPR editing...increases the probability that the locus of interest has also been edited! Thus, ouabain selection enriches...coselection are available at Addgene, and the Doyon lab has also provided a detailed protocol at Protocol Exchange...
Showing: 1 - 15 of 15 results