We narrowed to 254 results for: Cas9

...lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)...are enriched in the 20 nm radius surrounding the dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology... technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted spatial...

...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...

...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy...

... editing - all influenced by an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...

...CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit Disease Out of the genome Tips for a first...

... Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs...

...end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for editing...

...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

...) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled.... Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein....modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9 as a master...an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module...specific proteins via aptamer domains. Combined with dCas9, this new scaffold RNA (scRNA) will bind to a PAM-adjacent...layered with distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field...applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory...

...Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding...protein-bEFP fusion. Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the...

...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

... EGFP and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact...

... endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging ...

...changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the...

...primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org...

...used with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression...

...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even here...

... Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi: https...

...versions of non-modular proteins like Cre. Could Cas9-DOG be developed soon to allow genome modification...

...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...