We narrowed to 250 results for: Cas9
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TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m.... a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...miniCRISPR” version, are much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...
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8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...ancestral bias in CRISPR guide library design. dCas9-directed DNA Demethylation: Guest blogger Daniel... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader...promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi is stably... -
The 12 Days of CRISPR
TypeBlog PostPublishedJan. 3, 2023, 2:15 p.m....🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....editing in moderate thermophiles. These evolved SpCas9 variants have improved activity on NAG PAMS and... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...