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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 192 results for: FAST

Showing: 21 - 40 of 192 results

  1. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research... the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will...

  3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor Lower-stringency...resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct...The idea was to evolve a TadA-7.10 version with a faster deamination rate so that deamination can happen...PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems allow for rapid continuous...speed dictates which phage variants can propagate faster than the rate of dilution (passage from the infected..., they identified TadA-8e, which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 ...functional domains speed up the development towards faster and more precise base editors capable of editing...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...CRISPR plasmids New items from the viral service A fast blue-light inducible Cre for precise control of ... can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.  Sheets, MB...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...

  9. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...CRISPR material for you!  The CRISPR field moves fast. That’s why we’re bringing you a brand new third...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... to grow, clone, and manipulate their vectors in fast-growing bacteria first. In fact, most yeast vectors...you also have to consider how to keep it there. In fast-growing populations of cells, the vector needs to...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...design.   Optogenetics Chemogenetics Timing Fast activation/deactivation Prolonged activation Targeting...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same level of control...

  18. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed...
Showing: 21 - 40 of 192 results