We narrowed to 28 results for: cas12a

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ...opt for a completely different Cas effector, like Cas12a (Cpf1). Even more creative options involve generating...

2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...developed nucleic acid detections tools based on Cas12a and Cas13  which could be repurposed for SARS-CoV...

3. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...Updated Aug 13, 2020. Note: Cpf1 is also called Cas12a. In 2015, Zetsche et al. added to the CRISPR toolbox...

4. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers...

5. Build Your CRISPR Vocabulary

   Type

   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

6. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

7. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and...

8. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from...