...detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA...

...cell line groups, such as their vaccine production, SARS-CoV-2 research, or adenovirus packaging cell lines...

...that the majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry...

...Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi....

...Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while the early results were promising...

...researchers to share information about the emerging SARS-CoV-2 virus quickly and openly. By sharing results...bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger...

...CRISPR-Cas13 based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was...

...team, you don’t necessarily want a lot of redundant skills - you just want the necessary skills. You might...members are highly trained, and though there are necessary redundancies in skills, individuals also tend ... team So why is it helpful, or sometimes even necessary, to build a team in the first place? Let’s think... requires a broad knowledge base is going to necessarily need a diverse group of people to provide that... sense of purpose, there are many more things necessary to make a successful team. Entire books have been...roles your team requires to function is certainly necessary, but equally important is making sure all team... Even if you end up on some teams that don’t necessarily work well, you can learn from the experience ...

...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...opens its doors to the public, for example at an anniversary or for Girls’ Day, offer guided tours to show...

... replication initiation proteins (RepA, RepB) necessary for replication in various hosts (Pagatto and ... plasmid is conjugative and carries the genes necessary for transfer initiation between bacterial hosts...Providencia spp., Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus...Providencia stuartii, Pseudomonas spp., Rhizobium leguminosarum, R. trifolii, Salmonella enteritidis, S. typhimurium...fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella...

...but avoiding unnecessary future conflicts while giving people the tools to resolve necessary ones on their...data at a conference poster session, you don’t necessarily want everyone who visits your poster to agree...about how something should be communicated is necessary in a large organization, because confirming that... Is this level of domain knowledge absolutely necessary for conflict resolution? Definitely not. But it...

...permanent residence “green card” process is a necessary and often overlooked part of career development...residence. The recently established case Matter of Dhanasar provides the three-prong test that US Immigration...related to the foreign national’s field.” (Matter of Dhanasar) In your application you must be able to show ... the top people in your field. No employer is necessary and no job offer is needed for this category. ...found in skin cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship...

... experts, and instead convene the necessary groups for the necessary discussions and actions now.  Many...safe to manipulate an embryo—even if it’s not necessarily via CRISPR/Cas9. This leaves us with the second...whether or not we will stop shuffling our feet on necessary actions to review and decide on the future of ...

...These include Arch(D95N) and QuasAr, as well as the eFRET sensors, QuasAr-mOrange and MacQ-mCitrine. All...cellular processes. For a successful screen, it is necessary to identify the gRNAs that elicit the phenotype...to manipulate genetically. To solve this issue, Sarah Teichmann and colleagues created a retroviral CRISPR...

...to share them with you, we realized our 20th anniversary was about all the amazing years behind us, as...Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning Let’s take a quick...Production) pie-fully celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene...beyond! Which brings us to 2024, Addgene’s 20th anniversary and the year our repository grew to over 150,...

...a deep understanding of retroviral biology is necessary in order to use the vectors correctly. A solid.... The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation ... G. M., & Temin, H. M. (1974). Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. Journal...M. (1973). RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis with 70 S viral ...

...the minigenome contains all cis-acting signals necessary to direct minigenome replication and reporter ...vectors) because they do not encode all of the necessary viral proteins. Minigenome systems have been developed...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively ...transfected into cells. All of these components are necessary to efficiently reconstitute a functional viral...

...Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site, but now we were presented ...meant that we already had a clear idea of the necessary steps to perform:      Fig. 2: Quick-stepping...internal software infrastructure; however, it is not necessary to use containers with Nextflow. For each of the... in a cloud environment. Given the throughput necessary to keep up with our sequencing instrument (hundreds...

...pause during elongation which may actually be necessary for proper protein folding (Stadler and Fire, ...synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always...Santana-Quintero LV, Katneni U, Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource for codon usage.../doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous...

...voltage indicators: QuasAr1 and QuasAr2 (quality superior to Arch). Both QuasAr1 and QuasAr2 possess mutations...DNA-guided optogenetic tools; however, targeting the necessary light-activated protein domains to the appropriate...kinetics. By combining both the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch...Arch indicator (pJMK004), as well as the CheRiff, QuasAr, and Optopatch vectors available to the scientific...