We narrowed to 81 results for: sgRNA

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

April 7, 2015, 4:21 p.m.

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Nov. 29, 2022, 2:15 p.m.

Curious about how, exactly, Cas9 does what it does? Dive into the structural biology of Cas9 for an answer!

June 4, 2024, 1:15 p.m.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

May 12, 2026, 1:15 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

In our latest Hot Plasmids, we feature new systemic capsids, Cas-Page, an anti-FLAG antibody, and more!

Feb. 20, 2024, 2:45 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

This post highlights some of the tools Addgene makes available to industry scientists.

Jan. 13, 2026, 2:15 p.m.

Prime editing is a CRISPR/Cas9 editing method that offers more versatility and precision to genome editing. This post covers common prime editing tools.

Jan. 13, 2025, 9:49 p.m.

The Open Repository of CRISPR Screens is a resource to increase the accessibility of CRISPR screen data. Learn how to use this for your research.

Jan. 21, 2020, 2:30 p.m.

Learn all about a highly processive reverse transcriptase, a new nanobody toolkit, and CRISPR-based tools for monitoring protein-DNA interactions

June 27, 2018, 1:16 p.m.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

March 24, 2016, 2:30 p.m.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Feb. 2, 2016, 5 p.m.

An overview of viral applications spanning from basic research science to clinical therapies.

April 13, 2023, 1:15 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.