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We narrowed to 14 results for: sgRNA

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Showing: 1 - 14 of 14 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro... , David Root 247026 Human sgRNA library Jacquere in pMV_AA050 Human sgRNA library in backbone pMV_AA050...Doench , David Root 247027 Human sgRNA library Jacquere in pRDA_734 Human sgRNA library in backbone pRDA_734...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro... , David Root 247028 Mouse sgRNA library Julianna in pMV_AA050 Mouse sgRNA library in backbone pMV_AA050...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing...control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum ...mutations in RT enzyme PE3 — PE2 plus additional sgRNA PE4 — PE2 plus MLH1 (a protein component of MMR)...the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether...David Liu 140448 QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg Plant...BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia...172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian...

  4. CRISPR References and Information

    Type
    Collection
    ... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) A tool that... and a ctivation (Link opens in a new window) A sgRNA design tool for genome editing, as well as gene ...Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for... (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation for base ... window) Ranks and picks candidate CRISPRko/a/i sgRNA sequences to maximize on-target activity for selected...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral... promoter Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Dual-sgRNA_hU6-mU6 - Lentiviral expression plasmid for two sgRNAs from an hU6 and an mU6 promoter Protein-protein...confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice...3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral...

  6. Retrovirus Plasmids

    Type
    Collection
    ... safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...

  8. Plant Plasmids and Resources

    Type
    Collection
    ...Nicotiana benthamiana . Plasmids for expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification...

  10. Lentivirus Plasmids

    Type
    Collection
    ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

  11. CRISPR Guide

    Type
    Collection
    ...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... D. E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...Hyman 87200 pX335 HTT sgRNA-b HTT U6 Huntington's Mahmoud Pouladi 87201 pX335 HTT sgRNA-a HTT U6 Huntington's...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...
Showing: 1 - 14 of 14 results