We narrowed to 81 results for: sgRNA

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Oct. 13, 2022, 1:45 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

Learn about CRISPR terminology for common DNA, proteins, and RNAs involved in this CRISPR 101 blog post.

July 2, 2024, 1:15 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Feb. 24, 2015, 7:50 p.m.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Jan. 23, 2025, 2:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

Nov. 19, 2025, 2:15 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Sept. 21, 2021, 1:15 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Nov. 21, 2024, 1:30 p.m.

Curious about what's new in the Addgene repository this spring? Check out our latest Hot Plasmids post!

May 23, 2024, 1:15 p.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.