We narrowed to 468 results for: try

...cloning, Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...incubation, and amount of DNA. As a general rule, try to use an excess of insert compared to the backbone... fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial... you might want to reconsider your approach, and try a different cloning strategy. Once you've identified...

... seemed fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “...back to the beginning, reassess the situation, and try a new strategy. Step Five: Share your results.  ... and ask them to explain their logic. Or you can try applying the scientific method to everyday problems...room to improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself...

...be familiar with the specifics of your research. Try to avoid jargon in this initial introduction. During...something you missed during the conference, you can try getting up to speed on social media. If you haven... when you are presented with a sea of strangers. Try things like sitting at a table for lunch with people...networking happens everywhere! Even when you aren’t “trying to network.” For some more networking tips not ...

...Therefore, I try to stay away from the loaded and judgemental language. In each peer-review, I try to mention...first read At the first read of the paper, I usually try to grasp the main message of the work. I underscore...

...lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas is nothing new... a bit about recruiting scientists to outreach.  Try new things to further your career Take advantage ...departments Graduate school was an excellent time to try new things, develop new skills, and meet new people... the Indiana State Museum and others when I was trying to find local informal science educators, and ever... mine was geared toward careers in academia or industry. Luckily, I discovered that there are many ways...

...During my first year of college, I knew I wanted to try research, but had no idea what to expect. I had a...university to perform research   Why should I try research? Personally, I have always been a pretty... when I was 18. I just knew I was interested in trying research, and if that’s where you’re at too, it...important.  As you’re talking to people, though, try to be conscious of who has your best interests at... and ever-expanding. While it can be tempting to try to plan out a very distinct and certain path, embracing...next career stage (ex: grad school, med school, industry) Other roles you might see Lab manager: ...not limited to working at a bench in academia or industry, so feel free to explore! Addgene has an entire...

...this what you mean? Are you trying to say this?" I'd say, "No, I'm trying to say X." She would then look... how many times I say this, candidates regularly try to convince me that their search committee is different...across that is not covered, please email me and I'll try to answer your question and subsequently update the...

...buying and try to inversely pair brightness with abundance whenever possible.      Fig 2: Try to inversely...other signals. But PE can be very helpful if you're trying to find a relatively rare protein in an experiment...January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...

...you’ll identify your purpose in using that image. Try using the questions: What is the goal, who is my ...be different for them when I’m done? What are we trying to fundamentally change about their knowledge?    ...together Now that you’ve gone through this process, try writing your own alt text for the example image. ...

... a toss-up as to whether it’s faster to order or try to make it in your lab (but if you need a lot of ... no guarantee you’ll get a response on the first try (or any response at all). And while this method is...high-throughput” Instead of making one plasmid at a time, try constructing several plasmids in parallel. That way...

...about their needs and any problems that they are trying to solve. After learning more about the stakeholders...software product management. Early in your career: Try to get software product management internships, preferably...you can’t find internships at science companies, try to find internships with translatable skills: working...

...what the result was, so try not to do this. These are the simple rules that I try to follow in my own research... Can Use to Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources...

... if you take time when you need it, rather than trying to push through a state of panic. Now, if I feel...you, it’s noteworthy and memorable and awful. So try to keep some perspective. Most people won't notice...did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify the specific...

...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...backbone + insert, unsuccessful 2 and 3 above), you can try ligating the recipient plasmid alone in the presence...you are plating on the appropriate antibiotic and try varying the “recipient plasmid : insert ratio” in... Addgene Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids...

...priority: location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere...will be achieved. On the other hand, if you are trying to knock-in a patient mutation or need to make ...efficiency — although both are of course preferred! When trying to strike that balance, you may wonder: how close...

...science education Do you aim to reach young people? Try networking with your local education community. Get...ATJCagan or create agar art with colorful microbes? Try your hand at photography and participate in a science...Nothing prepares you better than learning on the job. Try to get an internship at a magazine or newspaper (...

...non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes and these...IF and if a protocol is available. When possible, try to start with conditions and reagents that have already...the only way to know if a method will work is to try it. As you embark on your next IF adventure, just...integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177...

...have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily...stop you from embarking on a fruitless journey of trying to get males to recombine two mutations onto the...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...looked clean, professional, and modern. Instead of trying to place all of the content above the fold, we ... of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...solution to the problem you called about. Next, you try an email, given that this is the 21st century and... direct service you’ve come to expect from us.   Try out the Help Center! The Help Center can be found...