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This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

Protocols for plasmid cloning by PCR.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for creating a glycerol stock, including additional tips for long-term storage.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

Follow this protocol to perform RNA extraction without a kit.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol - How to Ligate Plasmid DNA

Protocol for Inoculating a Bacterial Culture

In this protocol you will learn the basics of how to use a light microscope.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose response.

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.