We narrowed to 16 results for: try

...type and the protein of interest. You may need to try a variety of fixation methods to find the best conditions...

...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...colonies on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence... that your transformation worked. You could also try varying the amount of recipient plasmid to insert...

...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...colonies on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...you are plating on the appropriate antibiotic and try varying the amount of recipient plasmid to insert...

...glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock too many times...

... that you cannot perform your duties. Be sure to try out different sizes to know your fit. It is best ...

...instead of incubating at -20°C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 ...

...protect the UV box from being cut by the razor blade. Try to get as little excess gel around the band as possible...

...site (MCS), but does not cut your insert, you could try using two different enzymes that have compatible ...

...do controls. See Tips and FAQ below for details. Try different vector to insert ratios to optimize the...

...growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...

... a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust the position of...

... a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...

... purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM MgCl 2 and/or 1µl DMSO to each...

...location of the protein of interest. You may need to try a variety of lysis buffers to find the best for your...

...microfuge tube and vortex. Pro-Tip You may need to try a few different concentrations of antigen to determine...

...the closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing...