We narrowed to 256 results for: try

...cells (visualized via the FITC channel) and you are trying to see what percentage of total live cells are ... biorender.com.   In such cases, instead of trying to quantify based on percentage positive or negative...

...cleaning up a PCR product, a common material you are trying to remove from your sample is protein. Proteins....  If you see evidence of contamination, you can try re-purifying the sample you have, but if time and... better off starting from the beginning. Before trying again, revisit your purification protocol. Are ...

...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have found ...interest (Figure 1). It can therefore be helpful to try all three when looking to label an intracellular ...

...your video call.  Expect technical difficulties. To try and circumvent this, test out the video conferencing...

...these dyes, as this would create difficulty when trying to resolve those populations. PE/Cy7 and BB515,...

...your budget? Email [email protected] to try out the JF dyes. Acknowledgements I thank Brett ...

...that your gating strategy will be based upon. We’ll try to keep it short though, promise! FSC and SSC: scattering...

...This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam...

...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of...10–15 V/cm of gel), your gel may be overheating. Try running it in a cold room or putting ice packs in... an issue, which can happen with large proteins, try lowering the voltage and using a longer run time....weird issue and can’t figure out what’s causing it, try making fresh running buffer. Old or improperly made... in different pore sizes for different proteins: try using a 0.2 µm (psi) membrane for small proteins ...voltages.  If your small protein is over-transferring, try using the faster semi-dry transfer, which works better...

...time before the experiment. What fixed it was not trying even harder to start earlier, but simply moving...work harder - it's to work differently.  Tools to Try  Checklists Found in surgical suites, air fields,...physical copy on hand as you run your experiments. Try drawing out a protocol as you read it, or putting...you to use cold block systems or buckets of ice. Try finding ways to intentionally set up your bench to...for you to reduce the chances of making an error. Try a variety of things, including (and maybe especially...

... test them all, so how do you know which ones to try?  Read on and we will provide you with some advice...acid, for example in a Western blot. If you were to try and use such an antibody in an assay for a native... will bind to your primary antibody. If you are trying to visualize the precise location of a protein ...primary:secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western..., and other information to make sure you’re not trying to choose between the same antibody sold under ...

...you should give similar workshops a try if you’re ever looking to try out a new technique or reagent! ... bit more about high throughput DNA prepping by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep...technology. Then we split into pairs to actually try out the kit itself. This was great because it forced...

... a lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...faster than it can be officially regulated. I did not try to sugarcoat the potential dangers of people using...not. But seeing some students really get it, and trying to answer some of their great questions, was immensely...difficult-but-rewarding jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you...