Plan Your Experiment
Type
Guide
...limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service. Delivery Using...
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Antibody Guide
TypeGuide...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common... -
Molecular Cloning Techniques
TypeGuide...via a proprietary BP clonase, creating an entry clone. The entry clone now has recombined attL sites flanking...made an entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors...collection contains thousands of Gateway donor , Gateway entry , and Gateway destination vectors with different...reducing ligation efficiency. TOPO-ready Gateway entry plasmids are also available, allowing for rapid ... -
Molecular Biology Reference
TypeGuide...acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and purification...select or sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. Empty Backbones...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ... -
Promoters
TypeGuide...metabolic operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Constitutive Hybrid... The trp operon involved in the production of tryptophan is an example of a positively-controlled operon... -
Adeno-associated virus (AAV) Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood to the CNS...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Gamma-Retroviral Vector Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Adenovirus Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Lentiviral Vector Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
CRISPR Guide
TypeGuide...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (... & Liu, D. R. (2018). Base editing: precision chemistry on the genome and transcriptome of living cells...