... and communication. A great approach to fully understand and acquire competitive skill sets in a myriad...with a strong impact, or a journalist does not understand the high-level science discussed in an interview...your project may not advance beyond its initial stages. Help you build a network outside of the academic... incorporating EAs into your routine.  Getting started: Planning your career path and identifying needed...Science writing competitions (e.g. Access to Understanding)   Writing Communication Publicity ...

...out to start the conversation. Many researchers are open to sharing knowledge and establishing collaborations...perform research there temporarily. The length of stay could be from a month to a year. The researcher ...take good notes, and ask questions if you don’t understand something. Offer to help out wherever you can...a really good time! If it sounds right for you, start thinking outside of your current research box, send...

...profound, and this is a rare instance in which "profound" seems like an understatement. Multiple speakers from...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding topics...implant human kidney progenitors into a pig. In stark contrast to recent genome engineering technologies...

...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate a ribosome...silent. Both of these methods increase our understanding of translation dynamics, but they come with ...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

...individual fluorescent protein (brightness, photostability, and so on; see the previous blog post for ...CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. If you must... microscope. Ideally, you would like to have substantial overlap between the excitation and emission filters...most recently published ones), they can be a good starting point. In many cases it is sufficient to use a...screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma #49004) to...

... and your career ambitions. First, how does one stand out amongst a pool of successful applicants and ...the end of your PhD or contract, you should be starting to send out polite emails with your CV to people...with others to accomplish your goals. You must understand your own style before making this choice.  How...You won't always be in the zone, but even brief stays there can make all the difference. Choose a lab ...Achieve Success in Academia  Advice for Scientists Starting a Lab   ...

...function and a well established cellular signaling gene, thus providing a starting point for downstream...per gene approach that has been the historical standard. Specifically, in our recent publications, we ...profiling, systematic tumorigenesis assays, drug epistasis assays, and morphological profiling can be used...essence in clinical settings, looking ahead, we’re starting to build saturating libraries of all possible ...

...Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive for many labs...Since there are many thousands of partitions, statistical modelling can be used to determine the actual... measure of target abundance, does not require standard curves, and can theoretically detect a single ...reference probe designed to bind at an unedited site distal tothe predicted double strand break, and a NHEJ...individual target molecules based on their mutation status. The drop-off probe is designed to perfectly complement...

...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...represents Addgene’s largest new initiative since we started distributing plasmids in 2004. We’ve already distributed... 501(c)3 nonprofit and our plasmid service was started based on donations. That seed money was used to...Wyss Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral... allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder...

...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...Weinstein  Listen to the CRISPR podcast segment Understanding the connection between specific genes and an...an immune response has been a longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells ...non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune...

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA... different enzymes. CasPEDIA is meant to be a constantly updating database which serves as a resource ...That’s a lot of helpful information! To get you started, we’ll give a quick overview of each CasPEDIA feature...activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves ssDNA...the previous information may be all you need to start your targeting experiment. However, many situations...

...Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut...geneticists to study gene function. In this final installment, I’ll provide a brief introduction as to how ...flies? Sure, the vector will have an antibiotic resistance gene to select for transformed bacteria, but ...crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting, your flies...

...Department of Stem Cell and Regenerative Biology is to understand the basis for cardiovascular and metabolic human...question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence the disease...are the implications of this kit in terms of understanding human disease? Musunuru: The power here is that...which seem to contribute to a particular disease state. You could use the kit to insert all of those variants..., CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility...

...functions. It’s an important process that we need to understand so that we could possibly develop pharmaceutical...in women with TS. “The goal of our study is to understand why women with TS develop bicuspid aortic valve...regulate ion channels in the brain.  “I have a long standing interest in the biology of GPCRs and G proteins...nucleus,” says Dr. Terry Hébert, a professor and assistant dean of biomedical science education at McGill...to “expand our knowledge of GNB1 disorder by understanding affected signaling pathways and how they lead...

...performing RNA extraction. RNA is inherently not as stable as DNA- it is single stranded and its ribose groups...help to further streamline this process and/or stabilize RNA during tissue collection and homogenization...method.  Researchers have also developed ways to “stabilize” RNA within tissues before cell lysis. These products...cells or tissues- they don’t actually chemically stabilize the RNA molecules (Allewell and Sarma, 1974). ...RNAlater® with use of TRIzol® and ammonium sulfate stabilizing solutions can be made in house.   A common problem...

...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as...scientists. She, Kenneth Fan, and Benjie Chen decided to start an open science nonprofit that would facilitate ...transformed this process by aligning institutions with standardized agreements and electronic signatures to dramatically...Excellence Award for our electronic MTA system. We also started our blog and YouTube channel in 2013, which continue...

...been assigned an exciting new project aimed at understanding how a critical cellular pathway is regulated...application. Take immunohistochemistry (IHC), for instance. While an antibody may be validated for IHC, not... its target and consequently the quality of the stain. Choose the antibody that was successfully used ...independent antibody strategies that compare the staining patterns of multiple antibodies against the same...express a tagged version of the antigen and compare staining of the antibody to that of the anti-tag antibody...

...steps you can take to verify your own plasmids. Staying organized With hundreds of samples coming through...the first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every...inserted gene/functional mutations, antibiotic resistance, restriction sites used during cloning, and most... cloning sites. In the simplest case we have a standard backbone with one insert, and can use common primers...easier by selecting the right primer. This often starts by determining what features you expect to differ...

... at any edit distance. It won’t, however, compute any score other than the edit distance. Added functionality...each item changes from what the reviewers so painstakingly assessed to something a bit more representative... time to assess public response; and, it is the stage I think we are currently at in the CRISPR software...formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow the user to save...target sites, off-target sites, scores and the statistics that go into calculating them. For this, some...

...attention, practice, and honing of your skills from the start. Just like with any sport, hours of practice are...insight into an area - not just working within the established network of your peers. Furthermore, using new...excellence of the institution). Likewise, if you’re staying in the same lab where you have been working for... years, you need to be very clear that you are staying because it is the best place in the world for your... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality...