We narrowed to 807 results for: Cre

...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ...

...proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson lab for...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent...

...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

...critical thinking and problem solving abilities, increased my technical know-how, and helped develop my science...scientific knowledge and technical know-how that increased my autonomy and satisfaction with my work.  5....

...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary...

... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula...alternative science careers here Interested in creative science education tools? Check out this Addgene...

...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role?  I think the secret sauce can be summed up in one word – initiative...

... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming...

...1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha, 2006) and retroviral...AAV production yields had yet to be examined. Increased yields with sucrose supplementation is serotype...

...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all get the mentoring...that there’s some correct level of intimacy that creates the magic of a safe space. No matter why, now is...

...into the collection and to start shipping. They created the best safety protocols they could with limited...While many were sympathetic to this need, we're incredibly grateful that the Chan Zuckerberg Initiative ...

...empirical essays had also stirred some of my more creative faculties into motion, such that a desire to once...filmmaking, podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation...

...different biochemical secondary energy sources to increase protein yields from 36 μg/mL to 266 μg/mL. The...oxytetracycline enzymes. Next, this system was used to recreate natural biosynthetic pathways. Biosynthetic pathways...

...interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability...

...fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio five fold...scaffold and HA-binding regions, the Stasevich lab created a versatile tool that can be used to visualize ...