We narrowed to 66 results for: dcas9

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Feb. 13, 2025, 2:15 p.m.

Check out some great blog posts from Addgene Depositors.

Nov. 18, 2016, 3 p.m.

Learn all about how to regulate cell growth dynamics with optogenetics, the Riboglow platform for visualizing RNA production and localization, mCarmine, a new and improved red fluorescent protein, and the ACE reporter system for quantifying base-editing and Cas9 cleavage activity.

Sept. 18, 2018, 1:33 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

March 17, 2020, 1:32 p.m.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Feb. 21, 2019, 1:15 p.m.

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

June 12, 2018, 11:40 a.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

April 23, 2018, 2 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Sept. 21, 2017, 2:07 p.m.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Dec. 2, 2014, 7:23 p.m.

Curious about what's new in the Addgene repository this spring? Check out our latest Hot Plasmids post!

May 23, 2024, 1:15 p.m.