We narrowed to 67 results for: dcas9

1. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene....position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids...

2. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

   Type

   Blog Post

   Published

   May 17, 2016, 2:30 p.m.

   ...nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and searchable...

3. CRISPR 101: Cytosine and Adenine Base Editors

   Type

   Blog Post

   Published

   Feb. 13, 2025, 2:15 p.m.

   ...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...

4. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   Published

   Nov. 18, 2016, 3 p.m.

   ... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...

5. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC. To achieve...

6. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase...

7. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression ...

8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics...

10. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (CRISPRi), and...

11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules ...

13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the MLV Gag ...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ... mutations and tags, while a catalytically dead dCas9 can be used in conjunction with activator or repressor...

15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR ...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...tools. CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription...

17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or viral-mediated...

18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ... target RNA without cleaving the molecule (like dCas9) suggests that dCas13a may be useful for isolating...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

20. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific...