We narrowed to 67 results for: dcas9

...inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene....position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids...

...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...

... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...

...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC. To achieve...

...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase...

...with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression ...

...components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene...

...CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics...

...regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (CRISPRi), and...

...about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional...

...modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules ...

...Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the MLV Gag ...

... mutations and tags, while a catalytically dead dCas9 can be used in conjunction with activator or repressor...

...tools. CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription...

...samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR ...

...visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or viral-mediated...

... target RNA without cleaving the molecule (like dCas9) suggests that dCas13a may be useful for isolating...

... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific...

...of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...