Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
Type
Blog Post
Published
April 24, 2015, 2:39 p.m.
... primer on how Cre-lox recombination works.
Advances in Cre-lox recombination have included inducible...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...excision results in one of three recombinations, and subsequent recombinations cannot occur.
While the Brainbow...Brainbow-1.0 mice.) The combinatorial effect of multiple, independent recombination events leads to a rainbow...systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of the most... loxP sites to specify distinct patterns of recombination. In 2007, Joshua Sanes and Jeff Lichtman, both...M-CFP (membrane tethered cyan). Without Cre recombination, RFP is expressed. Cre can mediate one of two...