Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 192 results for: FAST

Showing: 61 - 80 of 192 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...is degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...

  3. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...acids. Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction...

  4. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...

  5. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...as well as improving delivery strategies to make fast, effective, and targeted therapy possible. This ...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...one. Though an initial response should be fairly fast, getting the full answer to a complicated question...responses asking for more specifics. We can help you faster if we get as much information as possible about...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the overnight...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM)...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...various manufacturers. These proprietary systems are fast (often <10 minutes) and simple, utilizing individually...fluorescent proteins. Chemiluminescence enzymes are cheap, fast, shelf-stable before activation, and light-insensitive...primary antibodies). Direct westerns are simpler and faster than indirect ones and can be used in highly quantitative...for very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...form a routine. Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages provided...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved with ... to the original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... environment, have a better understanding of the fast, deadline-driven pace of biotech. In other words...

  17. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...The four main FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes ...
Showing: 61 - 80 of 192 results