We narrowed to 84 results for: control gRNA

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Sept. 5, 2017, 1:58 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

June 2, 2016, 2:30 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

March 23, 2021, 1:15 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Aug. 28, 2025, 1:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

June 3, 2025, 1:15 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

May 5, 2020, 1:15 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.