We narrowed to 77 results for: sgRNA

...specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA, in human and mouse cells. They further develop ...

...PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...

...using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins." Nature communications 10.1 ...

...(SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done through...

...SapI yields 2 sites- the first site accepts the sgRNA target sequence for U6 promoter expression and the...

... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds...

... as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking...

...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

...the best when paired with the highest-efficiency sgRNA. The PAM sequence near this site is AGC, so in a...

...Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nat...

...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

...Shlyueva D, Müller I, Helin K (2016) Optimizing sgRNA position markedly improves the efficiency of CRISPR...

...28414855. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...

...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...

...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...

...sequence which could be adjusted by changing the sgRNA length. The system could be further refined by the...

... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...