We narrowed to 81 results for: sgRNA

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Aug. 6, 2024, 1:15 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene, including a miniature CRISPR-Cas systems, SEND plasmids, and prime editing improvements

Nov. 18, 2021, 2:15 p.m.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

April 23, 2018, 2 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Aug. 18, 2016, 2:30 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Sept. 16, 2025, 1:15 p.m.

Learn about hot new tools in our collection!

Feb. 17, 2026, 2:15 p.m.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

July 12, 2016, 2:30 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon