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...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...

...molecular cloning. Depending on whether you are trying to overexpress or knockdown a gene, you might need...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...how the scavenger hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded...

...nitrocellulose, you may decide you have nothing to lose by trying to strip and reprobe. In this case, we recommend...procedure, perhaps using longer incubation times, or try the stringent solution if you started with the mild...

...during protein folding, in which case you should try tagging the opposite end.  If neither placement works...protein of interest is expressed at a very low level, try a longer histidine tag to increase capture. Longer...

...send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone app either as ...suit, but this is not Facebook so no pets or kids. Try looking a bit over your shoulder for the picture....

...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...

... you. When embarking on a new literature search, try to find a recent review article to give you an overview...you are looking for a particular protocol or are trying to replicate an experiment, the methods section... you to our guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences...photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter @JLRoose....

...summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were the plasmid...

...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...

...not doing yourself or your science any favors by trying to go it alone. The help of fellow lab members .... If I invite someone to be on a project team, I try to make it very clear why that person is there. If...thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem...

... get a lot of office politics (many individuals trying to divert more resources towards their projects...modern genetics from the perspective of somebody trying to get hands-on lab experience is that the technology... may seem quite unusual but it has allowed me to try out working in many different sectors and to discover...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...months experience working in a developing tropical country. This is why I set up a placement working in a ...

...Experiments tend to be equally complex with users trying to probe multiple targets in parallel. In these...cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies that are all derived...Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. 977–983). ...

...currently available, you now face the challenge of trying to figure out what system might work best for your...streamlined approach, you might want to give Gateway a try. This method, developed by Invitrogen and based on...

...system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning...beginning an experiment, it is recommended that you try a few different ratios of transfer, envelope, and...

...first (and second) you won’t succeed, but you must try, try again. Now, this does not mean that you should...a different university, perhaps in a different country, choose where you go carefully. Funding agencies...

...started: Determine what type of outcome you are trying to achieve: Do you want to permanently knock out...to activate or repress gene expression? Are you trying to create a single point mutation? Do you want ...

...gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene...activation, repression, and barcoding.  3) Are you trying to target every gene in the genome, or a specific...

...and to get their answers to the questions. Don't try to get all panelists on the phone at once – you won't...knows some of the people in the audience. I always try to meet audience members before a panel discussion...

...to verify plasmids or validating your new clones, try these tips to make the most of your time and sequence... a number of sequence alignment tools available. Try these resources for lists of alternatives to BLAST...databases available are unlikely to have an exact entry for your favorite gene fused to an epitope tag or...