965 results

A brief summary of the history of plasmids and their use in cloning and molecular biology.

Oct. 29, 2015, 2:30 p.m.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

Oct. 27, 2015, 3 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

The Future of Research Symposium Boston 2015 aims to develop solutions to problems in academic research. Learn how you can get involved .

Oct. 20, 2015, 2:30 p.m.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Oct. 14, 2015, 2:30 p.m.

In this blog post, learn how Addgene tracks samples and how you could implement a similar system in your lab.

Oct. 6, 2015, 2:30 p.m.

This 3rd and final post of our Primer on Optogenetics has tips to help you design your own optogenetics behavioral experiment.

Oct. 1, 2015, 2:30 p.m.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Sept. 29, 2015, 2:30 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Sept. 22, 2015, 2:30 p.m.

In this 2nd post of our three post series on optogenetics, learn about the hardware you'll need to perform optogenetics experiments in mice.

Sept. 17, 2015, 2:30 p.m.

Addgene recently awarded $5000 in plasmids to rare disease researchers as part of the BeHeard Award. Learn more about the award here.

Sept. 15, 2015, 2:30 p.m.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

Sept. 10, 2015, 2:30 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

Learn how the team building atmosphere at Addgene keeps its employees excited about helping researchers share their science.

Sept. 3, 2015, 2:30 p.m.

A laboratory research management consultant and coach provides step-by-step advice on how to negotiate a budget when stating your lab.

Sept. 1, 2015, 2:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Learn about what it takes to start your own business in the biotechnology field.

Aug. 25, 2015, 2:30 p.m.

This blog posts details what it's like to be a grant writing professional and how to get into the grant writing profession.

Aug. 20, 2015, 2:30 p.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.