960 results

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

July 23, 2015, 2:30 p.m.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

July 21, 2015, 2:30 p.m.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

July 16, 2015, 2:30 p.m.

It pays for scientists to have a great resumes and CVs. Follow these simple steps to help get you and your resume noticed by future employers.

July 9, 2015, 2:30 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Learn how Tyler J. Ford, an Outreach Scientist at Addgene, transitioned from graduate school to working at Addgene.

July 2, 2015, 8:37 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

June 17, 2015, 9:04 p.m.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Tips for writing your scientific manuscript, including how to search for and organize papers.

May 5, 2015, 3:54 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Addgene's interviews Chris Voigt of MIT about the intersection of CRISPR and synthetic biology and his new work building circuits with CRISPRs.

April 22, 2015, 2:06 p.m.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

April 16, 2015, 3:45 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.