965 results

Addgene's Advisory Board helps steer and promote Addgene in its goal of sharing science. We welcome CRISPR expert, John Doench, to the Advisory Board.

Aug. 13, 2015, 2:30 p.m.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Aug. 11, 2015, 2:30 p.m.

This blog post explores ways to gain leadership experience at professional organizations like the National Postdoctoral Association.

Aug. 6, 2015, 2:30 p.m.

This blog post explores how commonly used GFP variants can undergo Green-Red photoconversion.

Aug. 4, 2015, 2:30 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

July 23, 2015, 2:30 p.m.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

July 21, 2015, 2:30 p.m.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

July 16, 2015, 2:30 p.m.

It pays for scientists to have a great resumes and CVs. Follow these simple steps to help get you and your resume noticed by future employers.

July 9, 2015, 2:30 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Learn how Tyler J. Ford, an Outreach Scientist at Addgene, transitioned from graduate school to working at Addgene.

July 2, 2015, 8:37 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

June 19, 2015, 3:08 p.m.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

June 17, 2015, 9:04 p.m.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.