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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 192 results for: FAST

Showing: 81 - 100 of 192 results

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...having high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...

  11. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ...ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... the most studied prokaryotic organism. With its fast growth rate, small size, and low cost, it’s understandable...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

  15. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...

  16. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...varies for different enzymes. Many companies now sell fast digest enzymes that can digest large amounts of ...

  17. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... find today are marketed for a specific purpose: fast growth, high-throughput cloning, routine cloning...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation)...and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them for a...
Showing: 81 - 100 of 192 results