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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 192 results for: FAST

Showing: 101 - 120 of 192 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...

  4. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...gene that is shared across all CRISPR types; the fast evolution of these adaptive bacterial and archaeal...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

  11. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

  12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  13. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ... viral DNA for quality control. This gives us a faster turnaround time than short-read sequencing, as ...The workflow converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools...program called Geneious. Within Geneious, the raw FASTQ reads are used for either guided or new de novo ...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...each other practice meeting people – compete for fastest to set up a coffee date or most cards collected...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative...30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...pandemic quickly and openly. By sharing results faster and more broadly, scientists can receive recognition...Preprints are all about sharing scientific knowledge faster and more openly — which is also our mission here...

  18. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...to mankind. And you probably understand how much faster it would be for an expert in the field to read ...students were fired up and enthusiastic and needed a faster presentation with more information than normal....

  19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... individually assembles the reads into a single FASTA sequence that our QC scientists can easily analyze...each sample. About 24 hours later we unload the FASTQ data from our instrument and begin the analysis....

  20. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts of... with a single size insert, liquid media can be faster and easier to work with. In either case, it can...
Showing: 101 - 120 of 192 results