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We narrowed to 515 results for: gan

Showing: 101 - 120 of 515 results

  1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

  2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...excitable cells of the nematode Caenorhabditis elegans. They have found several voltage biosensors and...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...may be due to different lipid compositions of C. elegans membranes, which might beneficially influence the...

  3. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...living organisms resulting in the emission of photons. The most familiar bioluminescent organism for most...the second most commonly used luciferase. Other organisms from which luciferases have been cloned from include...  Table 2: Properties of native luciferases Organism Peak emission (nm) Size (kDa) Substrate Cofactors...

  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ..., if you are studying a protein that may bind a ligand during your experiment, then you’d want to make...antibody that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody...part of your protein that may be masked while the ligand is bound, then you’ll get a false negative signal...Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological ...

  5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...

  6. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... of informatics. I am a molecular biologist who began coding for my thesis project a few years ago when...language favored by biologists. Almost as soon as I began, I realized that I needed a system to keep track... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...

  7. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA...angiogenesis (Witz et al., 2009). Then, scientists began focusing on the extracellular matrix and fibroblasts..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...here at Addgene! Along with other open-science organizations, we support scientists in sharing their results...

  10. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...

  11. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...interactions New auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service...Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly of multiple... the auxin inducible degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron...degron (AID) system has been extensively used in C. elegans since 2015 to conditionally degrade proteins of...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

  14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...and optogenetic tools exist, humans (like many organisms) are opaque, which makes most biomedical applications...There is a variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021...sometimes seen controversy, and the interactions of organic molecules like proteins with magnetic fields generally...magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...predictable kinetics over 14 hours of expression in C. elegans embryos. However, this initial timer was a tetramer...could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. Of course, timers may ...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...In April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric... amassed in places around the world, hypotheses began to develop that this illness was caused by either....73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & ...00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide epidemiology...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1 in addition...

  20. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...number of factors, including the age of the model organism, injection site, and titer. Addgene’s Viral Vector...the University of Pennsylvania’s Vector Core that began in 2018. By the end of 2023, our catalog had grown...respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors, which include calcium and...This prioritization happens for good reasons. We began offering our viral vector service because we realized...
Showing: 101 - 120 of 515 results