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We narrowed to 703 results for: mak

Showing: 101 - 120 of 703 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...requires that the RNA be tagged. “This tool makes it possible to make the protein specific to any endogenous...PUF proteins! A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins to hit...continue to evolve and improve, Abil says they will make those new plasmids available through Addgene, and...the final version,” she said. “If we learn how to make the library even better, we would be very happy ...

  2. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...people to take a break from their everyday tasks and make time for special projects? We borrowed an idea from...great success at accomplishing “we-should-really-make-time-for-that” type projects. Cross-team “Random...communication between labs. Scientists are often willing to make time for free food, and these lunches would provide...between labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I ...work to share time with the people around us. It makes the quality of our work better and keeps us smiling...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you...for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall...To expand the capabilities of Gibson assembly and make a more modular platform, the Jacks lab generated...cumbersome to use in a high throughput manner or make it difficult to quantify interaction strengths. ...Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...introduction of auxin range from 10-20 minutes, making AID an appealing tool for studying essential proteins...described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using CRISPR technology...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and antibacterial ...interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult (Ligasová...contamination when you are working without gloves or masks. Make sure to change the gloves and masks regularly instead...line stock for mycoplasma contamination before use. Make sure there is no contamination present in the serum...disposable pipettes to avoid cross-contamination. Make sure you keep all the required equipment and reagents...Avoid long exposure of the cell culture to air and make sure to tighten the flask cap before placing them...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate...articles (gasp). To my delight, not only was I able to make the edits without any reagent-grade endangered Martian...Vinculin is a loading control. 3. In terms of making the monoclonal line: I had never done it before...important results (for example, if you needed to make a figure with the data). Using acrylamide instead...agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene knockout was straightforward...who you are, you could quickly be on your way to making new scientific observations. References 1. Rago...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...distributed. Next month, we are making two changes to the policy that will make the process a bit easier and...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is...publication isn’t required to share your plasmids! You can make your deposit available before publication and we...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and...

  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...to update our web resources and plasmid pages to make them more useful and easy to navigate. We also updated.... Base Edit: Base editing plasmids allow you to make precise C:G->T:A or A:T->G:C edits in DNA without...Like RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue ...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...

  8. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ... and then condensing that and making it accessible to students.” Making science approachable is often ...eScience Labs.  “We work with faculty members to make sure the courses encompass what they want to teach...onsite, Caitlin can visit the operations team to make sure “that everything that’s going into the kit ...non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe to the Career Channel of the blog...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...this responsibility seriously. Every decision we make, every project we undertake and every service we... that our materials are affordable but enough to make us self-sustaining. With this unique model, we are...opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting our scientific...ever curious what specifically drives the decision making process at Addgene, just look to our core values...curated, high-quality data and reagents Addgene makes it easy for scientists to deposit and request plasmids...

  10. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... science brick-by-brick”. Our mission is to help make science mainstream and to show people the very human...at these struggles will bring people together and make these daily challenges a little easier to overcome... As we started building, we thought – how can we make the connection between LEGOs and science more concrete...and sounds to synchronize with the images thereby making the videos more real. To give you an idea of the...amount of work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO...

  11. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... programs and it seems to me that most Addgenies make an effort to avoid plastic bags and to conserve ...conservation in the lab One stingy science project was to make my own glass milk for purifying cut plasmid DNA ...amount of wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally high...replication machinery room to come in and get busy making copies.     Types of origins of replication ...plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic in high..., so if you need two plasmids for an experiment, make sure they have "compatible" ori's. See the table...more copies with the addition of chloramphenicol – make sure your growth conditions aren't working against...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ...been, a community resource that supports both toolmakers and tool users. We encourage you to share your...resource. We are excited to work with all of you to make this the best and most accessible recombinant antibody... are some ways that you can participate: If you make recombinant antibodies or other recombinant affinity...get started.  If you have suggestions about how to make the resource better or ideas for new materials to...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Gearing explains how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6.... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo....Your Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether...

  15. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...within the UV spectrum, while wavelengths over 700 nm make up the infrared (IR) spectrum. When photons are ...advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore... together in the same color panel. In this case, make sure to compare the excitation/emission spectra ...www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.   To make life easier, always try to use fluorophores with...worry, there are strategies to resolve this issue. Make sure to check out our blog post on color compensation...and 2) BV421 is a very bright fluorophore, which makes it easier to detect the transgenic protein. We don't...

  16. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...well-understood standardized terms makes it a lot easier for organizations to make policy decisions on research...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes.... Setting clear expectations from the beginning makes the sharing process smoother, more consistent, and...Stopwatch award and seek feedback as to how we can make our process easier for others.  If you’ve still ...

  17. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...the readability of your writing. It makes suggestions on how to make your writing less complex. While I...’s a list of tools and resources that might help make writing a little easier. Writing schedule tools ... should accept all of the suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s...and you can share your library with others which makes it easier to work with collaborators. Zotero is ...

  18. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...action. Instead of rushing in, you take steps to make it more likely you’ll get your tasks done correctly...to employers because they save time and money and make employees more productive. Careers where you’ll ...applicants compared to the number of available roles). Make your resume stand out Before you begin to apply ... need to think of other aspects of yourself that make you stand out. For every role you need to show that...Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your Dream Job Resources on Addgene.org...

  19. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...working toward a solution. At the end of the meeting, make sure to thank everyone for their participation and...through a meeting with no idea why you are there. Make the most of everyone’s time by considering the level...grows, so will the length of your group meetings. Make sure to keep them manageable. At Addgene, teams ...last meeting. Use some positive peer pressure to make sure everyone completes their assigned items to ...like to send around a Google doc where people can make comments. At meetings, we just focus on the parts...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...documentation helps make sure everyone is on the same page. Effective delegation allows you to make the best use...expected to DO all the work – she is expected to make sure the work gets done and done well. Involving...for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...mistakes will be made that could have been prevented.  Make sure everyone understands that no question is “dumb...
Showing: 101 - 120 of 703 results